Fig. 4. Activation of NFκB (p65) signaling pathway by uremic sera is prevented by antioxidant enzyme mimetics. ECs were incubated (15 min) with sera from control donors (C), and uremic patients (U) in the absence and presence of apigenin, genistein, quercetin, ebselen, EUK134 and EUK118 (n=4). A. Ratios of phosphorylated NFκB (p65) relative to the total protein. Data are corrected for cell number and are represented as Mean±SD. B. I?B was assessed by Western-Blot in ECs previously treated with ALLN (50 µg/ml, 30 min, 37°C). Images are representative of 4 different experiments. # p<0.01 vs control and *p<0.05, **p<0.01 vs uremia.